Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8w09
Q-score: 0.461
Li M, Yang R, Chen X, Wang H, Ghirlando R, Dimitriadis EK, Craigie R
J Mol Biol (2024) 436 pp. 168557-168557 [ Pubmed: 38582148 DOI: doi:10.1016/j.jmb.2024.168557 ]
- Wild-type hiv-1 intasomes (400 kDa, Complex from Human immunodeficiency virus 1)
- Vdna (8 kDa, DNA from Human immunodeficiency virus 1)
- Zinc ion (65 Da, Ligand)
- Integrase (32 kDa, Protein from Human immunodeficiency virus 1)
- Magnesium ion (24 Da, Ligand)
- Vdna (27 kDa, DNA from Human immunodeficiency virus 1)
- (4r,12as)-n-(2,4-difluorobenzyl)-7-hydroxy-4-methyl-6,8-dioxo-3,4,6,8,12,12a-hexahydro-2h-pyrido[1',2':4,5]pyrazino[2,1-b][1,3]oxazine-9-carboxamide (419 Da, Ligand)
Resolution: 3.23 Å
EM Method: Single-particle
Fitted PDBs: 8w2r
Q-score: 0.432
Li M, Yang R, Chen X, Wang H, Ghirlando R, Dimitriadis EK, Craigie R
J Mol Biol (2024) 436 pp. 168557-168557 [ Pubmed: 38582148 DOI: doi:10.1016/j.jmb.2024.168557 ]
- Dna (5'-d(p*cp*gp*gp*gp*ap*ap*ap*ap*tp*cp*tp*cp*tp*ap*gp*cp*a)-3') (7 kDa, DNA from synthetic construct)
- Dna (5'-d(*ap*cp*tp*gp*cp*tp*ap*gp*ap*gp*ap*tp*tp*tp*tp*cp*cp*cp*g)-3') (8 kDa, DNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Integrase (39 kDa, Protein from Human immunodeficiency virus 1)
- Hiv-1 p5-in intasomes core (Complex from Human immunodeficiency virus 1)
- Magnesium ion (24 Da, Ligand)
- (4r,12as)-n-(2,4-difluorobenzyl)-7-hydroxy-4-methyl-6,8-dioxo-3,4,6,8,12,12a-hexahydro-2h-pyrido[1',2':4,5]pyrazino[2,1-b][1,3]oxazine-9-carboxamide (419 Da, Ligand)
HIV-1 intasome core assembled with wild-type integrase, 1F
Resolution: 2.83 Å
EM Method: Single-particle
Fitted PDBs: 8w34
Q-score: 0.236
Li M, Yang R, Chen X, Wang H, Ghirlando R, Dimitriadis EK, Craigie R
J Mol Biol (2024) 436 pp. 168557-168557 [ Pubmed: 38582148 DOI: doi:10.1016/j.jmb.2024.168557 ]
- Integrase (32 kDa, Protein from Human immunodeficiency virus 1)
- Zinc ion (65 Da, Ligand)
- Hiv-1 wild-type conserved intasomes core (Complex from Human immunodeficiency virus 1)
- Magnesium ion (24 Da, Ligand)
- Dna (5'-d(*ap*cp*tp*gp*cp*tp*ap*gp*ap*gp*ap*tp*tp*tp*tp*cp*cp*ap*c)-3') (27 kDa, DNA from synthetic construct)
- (4r,12as)-n-(2,4-difluorobenzyl)-7-hydroxy-4-methyl-6,8-dioxo-3,4,6,8,12,12a-hexahydro-2h-pyrido[1',2':4,5]pyrazino[2,1-b][1,3]oxazine-9-carboxamide (419 Da, Ligand)
- Dna (5'-d(p*gp*tp*gp*gp*ap*ap*ap*ap*tp*cp*tp*cp*tp*ap*gp*cp*a)-3') (8 kDa, DNA from synthetic construct)
Single-particle cryo-EM micrographs of a chromatosome containing human linker histone H1.0
Zhou BR, Feng H, Kale S, Fox T, Khant H, de Val N, Ghirlando R, Panchenko AR, Bai Y
Mol Cell (2021) pp. 1-17 [ Pubmed: 33238161 DOI: 10.1016/j.molcel.2020.10.038 ]
Image sets:
Single-particle cryo-EM micrographs of a chromatosome containing human linker histone H1.4
Zhou BR, Feng H, Kale S, Fox T, Khant H, de Val N, Ghirlando R, Panchenko AR, Bai Y
Mol Cell (2021) 81 pp. 1-17 [ Pubmed: 33238161 DOI: 10.1016/j.molcel.2020.10.038 ]
Image sets:
Single-particle cryo-EM micrographs of a chromatosome containing human linker histone H1.10
Zhou BR, Feng H, Kale S, Fox T, Khant H, de Val N, Ghirlando R, Panchenko AR, Bai Y
Mol Cell (2021) 81 pp. 1-17 [ Pubmed: 33238161 DOI: 10.1016/j.molcel.2020.10.038 ]
Image sets:
Single-particle cryo-EM micrographs of 197bp nucleosome with scFv
Zhou BR, Feng H, Kale S, Fox T, Khant H, de Val N, Ghirlando R, Panchenko AR, Bai Y
Mol Cell (2021) 81 pp. 1-17 [ Pubmed: 33238161 DOI: 10.1016/j.molcel.2020.10.038 ]
Image sets:
Zhou BR, Feng H, Kale S, Fox T, Khant H, de Val N, Ghirlando R, Panchenko AR, Bai Y
Mol Cell (2021) 81 pp. 1-17 [ Pubmed: 33238161 DOI: 10.1016/j.molcel.2020.10.038 ]
Image sets:
Cryo-EM structure of a chromatosome containing human linker histone H1.0
Resolution: 2.93 Å
EM Method: Single-particle
Fitted PDBs: 7k5x
Q-score: 0.489
Zhou BR, Feng H, Kale S, Fox T, Khant H, de Val N, Ghirlando R, Panchenko AR, Bai Y
Mol Cell (2021) 81 pp. 166-182.e6 [ DOI: doi:10.1016/j.molcel.2020.10.038 Pubmed: 33238161 ]
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Chromatosome containing human linker histone h1.0 (Complex from human)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Histone h2b type 1-j (13 kDa, Protein from Homo sapiens)
- Dna (197-mer) (61 kDa, DNA from Homo sapiens)
- Histone h2a type 1-b/e (14 kDa, Protein from Homo sapiens)
- Scfv20 (29 kDa, Protein from Mus musculus)
- Histone h1.0 (20 kDa, Protein from Homo sapiens)
- Dna (197-mer) (60 kDa, DNA from Homo sapiens)
Cryo-EM structure of a chromatosome containing human linker histone H1.4
Resolution: 2.76 Å
EM Method: Single-particle
Fitted PDBs: 7k5y
Q-score: 0.527
Zhou BR, Feng H, Kale S, Fox T, Khant H, de Val N, Ghirlando R, Panchenko AR, Bai Y
Mol Cell (2021) 81 pp. 166-182.e6 [ DOI: doi:10.1016/j.molcel.2020.10.038 Pubmed: 33238161 ]
- Scfv20 (29 kDa, Protein from Mus musculus)
- Chromatosome containing human linker histone h1.4 (Complex from human)
- Histone h2b type 1-j (13 kDa, Protein from Homo sapiens)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Dna (197-mer) (61 kDa, DNA from Homo sapiens)
- Histone h1.4 (21 kDa, Protein from Homo sapiens)
- Histone h2a type 1-b/e (14 kDa, Protein from Homo sapiens)
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Dna (197-mer) (60 kDa, DNA from Homo sapiens)